引用:

Shia LaBeouf as Sam Witwicky
Megan Fox as Mikaela Banes
Josh Duhamel as U.S. Army Major William Lennox
Tyrese Gibson as USAF Master Sergeant Robert Epps
John Turturro as Seymour Simmons
Ramón Rodríguez as Leo Spitz
Kevin Dunn as Ron Witwicky
Julie White as Judy Witwicky
Isabel Lucas as Alice
John Benjamin Hickey as Theodore Galloway
Matthew Marsden as Graham
Glenn Morshower as USMC General Morshower
Matthew Yorlano as USMC Private Engraham (Extra)
Erin Naas as the Arcee/Chromia/Elita One rider
Rainn Wilson as Professor R. A. Colan
Deep Roy as an Egyptian guard
Voice acting:
Peter Cullen voices Optimus Prime
Mark Ryan voices Jetfire
Reno Wilson voices Mudflap
Tom Kenny voices Skids and Wheelie
Jess Harnell voices Ironhide
Robert Foxworth voices Ratchet
André Sogliuzzo voices Sideswipe
Grey DeLisle voices Arcee
Tony Todd voices The Fallen
Hugo Weaving voices Megatron
Charlie Adler voices Starscream
Frank Welker voices Soundwave, Reedman and Devastator
Calvin Wimmer voices Demolishor (credited as "Wheelbot")
John DiCrosta voices the Doctor
Michael York voices Prime #1
Kevin Michael Richardson voices Prime #2 and Rampage (credited as "Skipjack")
Robin Atkin Downes voices Prime #3